BALANCED SALTS AND BUFFERS

| ALSEVER | EARLE's Salts | HANKS's Salts | HEPES | PBS | Sodium Bicarbonate | Ultra-Pure Water|

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ALSEVER SOLUTION

Storage: +2 - +25^oC

Formulation (in mg/I)

NaCl	4200	Citric acid•H2O	550
Sodium citrate•2H2O	8000	D-glucose	20500

	Cat.No.	Unit
Alsever solution, pH 6.1	L6015	500 ml

EARLE'S AND HANKS' BALANCED SALTS

Storage: +2 - +25^oC

Formulations (in mg/I)

References:

1.Earle, W.R. et al., J. Nat. Cancer Inst. 4, 165 119431

2.Hanks, J. H. and Wallace, RE. Proc. Soc. Exp. Biol. Med. 71, 196 [1949]

3.Dulbecco, R. and Vogt, M. J. Exp. Med. 99, 167 [1954]

	PBS	Earle's	Hanks'
	(Dulblecco)	Salts	Salts
NaCl	8000	6800	8000
KCl	200	400	400
Na2HPO4	1150	-	48
NaH2PO4•H20	-	140	-
KH2PO4	200	-	60
MgCI2•6H20	100	-	-*
MgSO4•7H20	-	200	200*
CaCl2	100	200	140
Glucose	-	1000	1000
Phenol red	-	10	10
NaHCO3	-	2200	350

* In the original formulation, 100 mg/l MgCI₂ x 6H₂O and 100 mg/l MgSO₄.7H₂0 are indicated.

PBS PHOSPHATE BUFFERED SALINE (DULBECCO)

Storage: +2 - + 25⁰C

	Cat. No.	Unit
Phosphate Buffered Saline	L1815	500 ml
PBS solution
Phosphate Buffered Saline	L1825	500 ml
PBS solution	L1820	1000 ml
without Ca^2+, Mg ^2+
Phosphate Buffered Saline
(10x) PBS solution	L1835	500 ml
without Ca^2+, Mg ^2+
Phosphate Buffered Saline
PBS powder substance	L182-02	1l
without Ca^2+, Mg ^2+	L182-05 L182-10	5l 10l
	L182-50	50l

	Cat. No	Unit
Earle's saline (EBSS)
with 2.2 g/l NaHCO3	L1915	500 ml
Earle's saline (EBSS) (l0x)
without NaHCO3	L1925	500 ml

	Cat. No.	Unit
Hanks' saline (HBSS)
with 0.35g/lNaHCO3	L2015	500 ml
Hanks' saline (HBSS) (l0 x)
without NaHCO3	L2025	500 ml
Hanks' saline (HBSS)
without phenol red
with 0.35 g/l NaHCO3	L2035	500 ml
Hanks' saline (HBSS)
without Ca^2+, Mg^2+
without phenol red	L2045	500 ml
with 0.35 g/l NaHCO3
Hanks' saline (HBSS)
without Ca^2+, Mg^2+
with phenol red	L2055	500 ml
with 0.35 g/l NaHCO3
Hanks' salts - powdered substance	L201-01	1 l
without NaHCO3	L201-05	5 l
	L201-10	10 l
	L201-50	50 l

HEPES-BUFER

Storage:+2 - +25^oC

	Cat.No.	Unit
HEPES buffer (1 M) (50 x)	L1613	100 ml
HEPES (powdered substance)
(hydroxy-ethylpiperozine-ethane sulphonic acid)	L1603	100 g

How to use HEPES buffer

· Dissolve powder medium, adjust pH at 7.2 with sodium hydroxide.

· Add 10 - 20 ml of HEPES buffer per litre of medium (for delicate cell cultures, use only 10 ml).

· A concentration of 20 mM HEPES requires 20 ml/l medium, for 25 mM use 25 ml/l.

· If NaHCO₃ is required_, only add 10%.

SODIUM BICARBONATE

Storage: +2 - +25^oC

	Cat.No.	Unit
Sodium bicarbonate (NaHCO3)	L1713	100 ml
(7.5%)
Sodium bicarbonate - powdered	L1703	100 g
(NaHCO3)

ULTRA-PURE WATER

Storage: +2 - +25^oC

BIQCHROM ultra-pure water is processed in a multistep process to comply with the specification 'Water For Injection' (WFI). Initial purification includes charcoal adsorption and ion exchange methods. Final distillation uses a cyclone device for water of extreme purity. The water vapour is accelerated to 500 g through cyclone geometry and difference in pressure. Thus the vapour is cleaned of all dragged impurity droplets < 0.5 pm. The distillate is pyrogen free.

The quality of the ultra-pure water is regularly tested by

means of HPLC, (UV detector 220 nm and 254 nm, 0.02 AUFS; 250 mm x 4.6 mm, 5 μm, RP-column; linear gradient from 100% water to 100% acetonitrile).

	Cat.No.	Unit
sterile	L0015	0.5l
sterile, suitable for HPLC	L0020	1 l
in plastic canisters, not sterile	L0030	5 l
in plastic canisters, not sterile	L0040	10 l